Think Tank on Use of Metabolomics in Population-Based Research
September 27, 2012
6001 Executive Boulevard
Rockville, MD 20852
Metabolomics is a "top-down" systems biology approach to metabolic profiling, defined as the quantitative measurement of the dynamic multiparametric metabolic response of living systems to pathophysiological stimuli or genetic modification. The concept of metabolic phenotyping was initially introduced and developed to help explain the biochemical consequences of genetic variation in whole organisms and has emerged as a powerful new approach to augment genomic, proteomic, and transcriptomic methods for the capture of biomarker information on a range of toxicological and disease processes.
Until recently, metabolomics and other post-genomic platforms such as proteomics and transcriptomics have not been suitable for large-scale, high-throughput epidemiologic applications. Studies that employed metabolomics technologies have focused on toxicological, physiological, and disease responses in animal models and small-scale human studies. This has been due mainly to the limited capacity of the analytical platforms for sample throughput and the processing requirements for the enormous amounts of data created. Improved sample preparation (using automated liquid handling workstations), robotic sample-delivery systems, automated data processing, and use of multivariate statistical and chemometric methods, with associated reductions in cost, now are realizing the potential for metabolic phenotyping in epidemiology. Investigators have begun to extend these studies to larger scale population studies for biomarker discovery. With these studies comes the challenge of applying metabolomics technologies in a manner that generates meaningful results. Epidemiologists must strive to comprehensively understand the principles of metabolomics to determine when it is appropriate to use biomarkers identified using this technology, which includes the ability to determine when biomarkers have been validated sufficiently.
This Think Tank will bring together several key players in this emerging research area to share and discuss the state of the field of metabolomics in epidemiology research. The meeting will highlight the research of six metabolic experts who can shed light on how to apply this methodology to large epidemiology studies. The meeting also will be an unprecedented opportunity to hear from attendees who focus on a scientific agenda in the field of metabolomics and are interested in applying this technology to population science studies. Breakout group sessions will allow attendees to answer key questions to establish the current state of the field and help shape strategies to move this technology toward use in population-based research.
|8:15 a.m. – 9:00 a.m.||Registration|
|9:00 a.m. – 9:10 a.m.||
Welcome and Introductions
|9:10 a.m. – 9:40 a.m.||
Metabolomics of Cardiometabolic Diseases: Examples and Challenge
Introduction: Pothur Srinivas, Ph.D., M.P.H.
|9:40 a.m. – 9:50 a.m.||Discussion|
|9:50 a.m. – 10:20 a.m.||
Processing and Integrating Data from Metabolomic Platforms for Cohort Studies
Introduction: Joshua Sampson, Ph.D.
|10:20 a.m. – 10:30 a.m.||Discussion|
|10:30 a.m. – 10:50 a.m.||Coffee Break|
|10:50 a.m. – 11:20 a.m.||
Untargeted Metabolomics Approaches in Population Studies: Promises, Workflows, and Pitfalls
Introduction: Sharon Ross, Ph.D., M.P.H.
|11:20 a.m. – 11:30 a.m.||Discussion|
|11:30 a.m. – 12:00 p.m.||
Metabolomic Profiling in the Framingham Heart Study
Introduction: Padma Maruvada, Ph.D.
|12:00 p.m. – 12:10 p.m.||Discussion|
|12:10 p.m. – 12:25 p.m.||
Summary: Opportunities and Challenges
Introduction: Holly Nicastro, Ph.D.
|12:25 p.m. – 1:30 p.m.||Lunch|
|1:30 p.m. – 2:00 p.m.||
Metabolomics Research at NIH
Introduction: Joseph Su, Ph.D., M.P.H.
|2:00 p.m. – 2:10 p.m.||Discussion|
|2:10 p.m. – 4:00 p.m.||
Goals and Opportunities for Moving Metabolomic Technology to Population-Based Research
Integration of Data for Metabolomics Studies
Resources and Infrastructure Needed to Support Metabolomics Studies in Population-Based Research
|4:00 p.m. – 4:15 p.m.||
|4:15 p.m. – 5:15 p.m.||
Breakout Session Debriefing and Discussion
|5:15 p.m. – 5:30 p.m.||
Think Tank Wrap-Up
- Zanetti KA, Mette E, Maruvada P, Milner J, Moore SC, Nicastro HL, Ross SA, Sampson JN, Verma M, Su LJ. The future of metabolomic profiling in population-based research: opportunities and challenges. J Anal Bioanal Tech. 2014 5(4),
For further information or questions, contact Krista Zanetti, Ph.D., M.P.H., R.D.